Comparison of the Effect of Microwave Radiation in the Disinfection Complete Dentures Contaminated with Two Types of Bacteria S.aureus and P.aeruginosa (in vitro) with Mechanical and Chemical Methods of CoregaTablets

Introduction and goal: various pathogens colonized in oral cavity that cause systemic disease such as pneumonia, endocarditis, and chronic obstructive pulmonary disease. Denture contamination with pathogenic microorganisms considered as a potential source of contamination between patients and dental staff. The study aimed to compare the effect of microwave radiation in the disinfection complete dentures contaminated with S.aureus and P.aeruginosa in vitro using mechanical methods and chemical onebyCoregatablets. Methods: 72 dentures with standard techniques prepared and disinfected in an autoclave (121 c ○, for 20 minutes) was performed and 30 samples were considered for each bacteria, which 6 samples were in each subgroup assigned. 6 denture was used as a negative control (n =6) (Group 1). The rest of them, individually with S.aureus and P.aeruginosa inoculated (for 48 hours, were kept in the incubator 37c ○) and then were sterilized by one of the following methods. Chemical disinfection using coregatablet (water-soluble 37 C ○ for 15 minutes) (n = 6) (Group 2), mechanical disinfection by brushing dentures for 5 minutes (n =6 ) (Group 3), microwave irradiation in W 650for 3 minutes (n =6) (group 4) and the positive control group did not receive any disinfection method (n = 6) (group 5). μl 25 suspension in dilutions 3-10 to 6-10 were cultured in Nutrient Agar. Colonies after incubation (37 c ○ for 48 hours) were counted (cfu / ml). To assess the long-term disinfection, containers include Nutrient Broth and dentures in 37 c ○ were kept for 7 days and its opacitieswere investigated. Data were analyzed using one-way ANOVA and T-test. Results: There was no evidence of growth in 48 hours and no opacities in containers Nutrient Broth samples after 7 days of incubation dentures by microwave radiation disinfection (650 W, 3 minutes andCorega tablets and statistically in comparing with positive control group was statistically significant (P=0) in dentures disinfection by mechanical methods (brushing) microbial growth after 48 hours, the opacities was observed in all the dishes created Nutrient Broth and statistically was not significant in comparison to the positive control (P>0.05). Conclusion: microwave radiation (650 W, 3 minutes) and Coregatablets were completely sterile in the short term and long termagainst S.aureus and P.aeruginosa.